Roche lightcycler 480ii384 pcr w thermocycler 96 s 2. Lightcycler 480 system from roche view supplier profile. Roche molecular biochemicals lightcycler operators manual. Service manual light cycler please note that this is only. Roche lightcycler 96 biotecon diagnostics biotecon. The 96well block for the f plate format or the 384well block for g plate format. Use of the roche lightcycler instrument in a realtime pcr. Work economically, adapting workflows to your format and throughput needs. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples. Conveniently define or edit sample information in plate or table views. Roche completes lightcycler product portfolio with. Compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis, mirna research, and relative quantification of target genes. Benefit from a large, intuitive touchscreen interface. Eurofins dna quantification and snp detection on gold standard platforms.
Review and cite roche lightcycler protocol, troubleshooting and other methodology information contact experts in roche lightcycler to get answers. Use of the roche lightcycler instrument in a realtime pcr for trichomonas vaginalis. The main switch is located at the back of the instrument. You can now create experimental protocols more quickly by using templates and macros.
Data were analyzed with lightcycler software by the fit point method to minimize noise and obtain the best correlation coefficient possible between standards. Other areas include viral infections, metabolic, central nervous system disorders and inflammatory diseases. The roche lightcycler 480 realtime pcr system is a 96 wellplate based realtime pcr platform that is used for highly accurate qualitative and quantitative detection of nucleic acids and genotyping. The problem is, i have limited amount of rna sample but i have many genes to assay for. Roche roche signs exclusive agreement with itis life. The innovative lightcycler 96 application and instrument software creates a simple analysis workflow with easy import and export functions, email notifications. The novel platform, which utilizes a proprietary 1536well plate, is.
Roche this week launched the lightcycler 96 realtime pcr instrument, the latest in the companys line of lightcyclerbranded qpcr systems for life science research the new instrument incorporates many of the best features of roches existing lightcycler systems, and closes a very important gap in the lightcycler portfolio by offering researchers a platform in. Comprehensive programming options, a large touchscreen, and intuitive analysis software make it easy to obtain. Roche has brought many highly effective drugs onto the market and is a world leader in innovative cancer drugs. Roche diagnostics lightcycler 480 plate 96 white lightcycler.
Turn on the computer linked to the instrument and log into window xp. I am planning to run semiquantitative real time pcr assays in 96 well plates on a roche lightcycler 480. The technology is widely used in nucleic acid quantification, gene expression profiling, and detecting genetic variations. Realtime pcr lightcycler instruments roche molecular diagnostics has introduced the lightcycler instruments, the fastest thermocyclers with online monitoring of pcr based on fluorescence detection. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. Available with 96well low profile and 384well formats. Realtime ready custom qpcr assays can be selected for any human, mouse, or rat targets of your choice. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is. What if you had the power to get your qpcr data fast, reliably and with less effort. Ideal for all levels of experience, the lightcycler 96 instrument provides an optimal combination of temperature homogeneity and data reproducibility. The lightcycler 480 system is a highperformance, flexible throughput qpcr platform interchangeable 96 or 384well blocks that. Discover this compact and smart new member of the roche lightcycler realtime pcr system. Service manual light cycler please note that this is only a. Has anybody used the light cycler 480 system by roche.
Evaluation of two commercial realtime pcr assays for. Roche today announced the global launch of new software updates, including advanced high resolution melting analysis and qualitative detection modules for the lightcycler 96 and new support features for the lightcycler nano system. Lightcycler technology is the most innovative and rapid possibility for carrying out and simultaneously evaluating pcr experiments. Roche lightcycler 480 ii real time pcr system for 96 well plates. Roche launches new software updates for its lightcycler 96. With a total of 6 detection channels, this compact cycler offers the highest flexibility and allows single color and multiplex testing. Additionally, the equipment and software installations, including application training, are conducted by our knowledgeable staff. Intuitive touchscreen and powerful analysis modules. Roche diagnostics 04729692001 lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils. Warranty information on warranty conditions are specified in the sales contract. Roche product information related to the us market. Realtime ready assays are designed using the proprietary probefinder software, which is based on optimized and verified algorithms. Speed time to results with advanced yet easytouse software designed for both novice and experienced users. Jan 31, 20 lightcycler 96 realtime pcr system from roche life science duration.
Roche lightcycler 480ii384 pcr w thermocycler 96s 2. Roche lightcycler 480 realtime pcr run setup instructions. Lightcycler 96 system 5 lightcycler 96 system software as smart and intuitive as you want it to be whether you are a qpcr novice or a seasoned expert, the lightcycler 96 software can accommodate your needswithout wasting your time learning a new software package. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications. Smallest reaction volume with roche lightcycler 480 96well. Roche this week launched the lightcycler 96 realtime pcr instrument, the latest in the companys line of lightcycler branded qpcr systems for life science research the new instrument incorporates many of the best features of roche s existing lightcycler systems, and closes a very important gap in the lightcycler portfolio by offering researchers a platform in the midprice segment of. Jun 06, 20 roche today announced the global launch of new software updates, including advanced high resolution melting analysis and qualitative detection modules for the lightcycler 96 and new support features for the lightcycler nano system. Compatible to run on any roche or nonroche qpcr insrument. Maybe the downside is the lightcycler software that is. Use the upper right quick search via catalog number or product name.
So in order to complete the experiment with what little rna that i have, i am thinking of scaling down the volume of my pcr reactions. Smallest reaction volume with roche lightcycler 480 96. The lightcycler 96 instrument is a realtime pcr system for rapid cycling up to. All assays are functiontested and enable fast and efficient expression profiling of your genes of interest.
The result of more than a decade of roche experience developing highperformance qpcr systems is now accessible for any research lab. Use of the roche lightcycler instrument in a realtime pcr for trichomonas vaginalis in urine samples from females and males. Speed time to results with advanced yet easytouse software. In the us our pharmaceuticals are marketed by genentech. Roche lightcycler 480 garvan institute of medical research. Connection via network or usb stick, remote data analysis via email. Conforms to the european directive for in vitro diagnostic medical devices 9879ec in combination with the lightcycler 2. The lightcycler 96 is a realtime pcr cycler for medium sample throughput. Multicodertx system instrument setup and data export for the roche lightcycler 1.
The lightcycler 480 software uses unique roche algorithms for highly accurate and robust automated data analysis. Smallest reaction volume with roche lightcycler 480 96well plate format apr302011 hi, i am planning to run semiquantitative real time pcr assays in 96well plates on a roche lightcycler 480. The software is compatible with the regulations for electronic records subpart b. Quantification cycle values were extracted with the lightcycler 480 software version 1. The lightcycler 96 system delivers data that makes you a. Contact your roche representative for further information.
Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96. Roche diagnostics 04729692001 lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils catalog no. Maybe the downside is the lightcycler software that is somehow. Roche lightcycler 480 realtime pcr run setup instructions for rt2 profiler pcr arrays. Upload files that contain software or other material protected by intellectual property laws or by. Quickly start experiments from readytouse macros or templates for all applications.
Building on over 30 years of industry experience, roche custombiotech uses the powerful multidisciplinary skills found in roche facilities across the world. The roche lightcycler 480 realtime qpcr system meets a wide variety of applications such as array validation, snp analysis, haplotyping, geneknockdown studies, mutation scanning, and detection of nucleic acids. Combining the rigidity of a polycarbonate frame with thin walled polypropylene wells, the armadillo plate provides superior thermal cycling performance under all con. For more than 10 years, roche has repeatedly set the standard for realtime pcr competence with the lightcycler instrument series and its broad capabilities for rtpcr assays, analysis software and reagents. In addition, roche diagnostics has commercialized the cobas 4800 system, including the cobas z 480 analyzer developed for in vitro diagnostic ivd applications, and has recently developed a userdefined workflow udf software that allows the use of the cobas z 480 analyzer as a realtime pcr instrument. Combining the rigidity of a polycarbonate frame with thin walled polypropylene wells, the armadillo plate provides superior thermal cycling performance under all conditions without warping. Lightcycler 96 realtime pcr system from roche life science duration. Roche lightcycler 480 ii inquire about availability includes computer loaded with lightcycler 480 software release 1.
The versatile, platebased lightcycler 96 system helps you achieve fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and snp analysis compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis. The team offers products and solutions for manufacturers in the diagnostics and pharma biotech industry ranging from raw materials to system solutions for bioprocessing. System component 1 lightcycler instrument sample carousel for o 1. Armadillo pcr plate, 96well, clear, semiskirted, low. The lightcycler 480 system is a highperformance, flexible throughput qpcr platform interchangeable 96 or 384well blocks that provides a platebased, highly adaptable and versatile realtime pcr system for gene expression analysis, snp genotyping, and mutation scanning via high resolution melting hrm. Open experimental file on lightcycler 96 user software. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. Roche completes lightcycler product portfolio with launch.
New innovative lightcycler 96 application and instrument software creates a simple analysis workflow with easy import and export functions, email notifications. Quantification cycle values were extracted with the lightcycler 480 software version. It offers fast and accurate analysis that impresses with its intuitive and userfriendly software. Thermo scientific armadillo pcr, clear semiskirted, low profile, clear well plates are the ultimate in high performance pcr plastics. Software will calculate the standard curve slope, efficiency, error, r2, and yintercept relquant if not already done open experimental file on lightcycler 96 user software. Lightcycler roche life science realtime ready overview. The 96 well block for the f plate format or the 384well block for g plate format. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user. Apr 07, 2015 multicodertx system instrument setup and data export for the roche lightcycler 1. Innovative design of optics and thermal block provide an ideal combination of accuracy, temperature homogeneity, and reproducibility for unbiased result research requires. Accurate results expected from a lightcycler system even with a gradient.
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